Fig. 1. Deletion of miRNA-22 attenuates diet-induced obesity in female mice. (A) Analysis of body weight gain in WT and miR-22 KO females fed a chow diet or obesogenic diet for 16 weeks (n=7-14). (B) Percentage of body fat mass evaluated by nuclear magnetic resonance imaging (n=7-12). (C) Subcutaneous adipose tissue weight normalized by tibia length (SAT/TL); (D) Retroperitoneal adipose tissue (RAT/TL); (E) perigonadal adipose tissue (PAT/TL) (n=7-12). (F) Analysis of adipocyte area in PAT using hematoxylin eosin staining and (G) quantification of adipocyte area evaluated by ImageJ; bars 100µm (n=3-4). (H) UCP1 levels in PAT evaluated by western blot (n=3-4). * WT obesogenic diet vs WT chow diet (p<0.05); # KO obesogenic diet vs KO chow diet (p<0.05); & KO obesogenic diet vs WT obesogenic diet (p<0.05).